ANTIBODY SERVICES BY APPLICATION / ELISA ASSAY DEVELOPMENT

ELISA Assay Development

Are you in the process of designing an antibody-based kit or a diagnostic assay? Capra Science assists clients in developing reliable antibodies become key components of ELISA procedures. The basis of generating a robust ELISA for R&D, pre-clinical or clinical and veterinary diagnostics, lay mainly in the performance of the antibodies.

Steps of production

When generating an antibody for an immunodiagnostic assay, of most important steps are:

  1. Choosing the right antigen. Whole or part of a recombinant protein, synthetic peptide or DNA.

  2. The antigen being of high purity. Impurities generate antibodies against the unwanted molecules, that can give unspecific background in the assay. Capra Science has expertise in eliminating antibodies against impurities, if needed.

  3. Choosing the best host/-s for the ELISA setup. In some ELISA set ups, it is beneficial to have a catcher and detector antibody from different hosts.

  4. The strategy and excecution of the affinty purification of antibody. A well-structured outline of the purification and a professional execution is key to a well-functioning antibody. This is one of Capra Science’s many strenghts.

  5. Using the antibody at the correct working dilution. Our QC-analysis of your ready-to-use antibody, will give you directions to its working dilution.

Capra Science Antibodies can assist you with developing the crucial antibodies that will drive your R&D or product development forward. Within the assay, some specific antibodies are usually conjugated with radiolabel, flourescent label, color-forming enzymes, or other molecules for detection. We are able to provide you with your antibodies in conjugated and non-conjugated forms.

Suitable host

Antibodies made for ELISA are polyclonal and/or monoclonal. The host depends on the particular setup of the assay platform.

Why produce your ELISA-antibody with Capra Science

  • Our advanced laboratory, in-depth knowledge and robust protocols guarantee the best performing antibodies.

  • Your project and intellectual property is safe with us. No subcontractors.

  • Capra Science holds an ISO9001 quality assurance to guarantee the best possible antibody quality.

  • Project reports, certificates (CoA), timelines and results are communicated throughout the project.

  • Our ethical standards are of the highest priority.

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Top 7 reasons to work with us

  1. Expertise. Our advanced laboratory, in-depth knowledge and robust protocols guarantee the best performing antibodies. The antibody expertise of our staff will be within your reach throughout your project.

  2. Discretion. Your project is safe with us. The whole chain of events in your antibody production and downstream processes are done at Capra Science’s own farm and laboratories with no subcontractors.

  3. Quality. Capra Science holds an ISO 9001 quality assurance to guarantee top product quality.

  4. Cost-efficiency. Our unique animal care program has been optimized to yield a high titer antiserum.

  5. Ethical standards. Animal welfare is our top priority. We run 1st class award-winning animal care facilities at our purpose-built organic farm.

  6. Flexibility. Your antibody production is customized to meet your specific needs.

  7. Personal service. A project with us includes free expert email consultation throughout your antibody production. We enjoy the personal contact with every client. Our scientific curiosity and nerdy ambition to continuously learn and improve, make us good listeners and allow us to deliver exactly what you need.